Unprecedented antibody localization
Using antibodies available from MitoSciences (Eugene, OR) for a mitochondrial membrane protein (porin) and a matrix protein (Complex IV subunit I) we have shown clear resolution of the mitochondrial matrix and membrane. The sample is a liver biopsy counterstained with DAPI. The secondary antibody for porin is Texas Red and that of complex IV subunit I is FITC. The resulting images show large blue nuclei and the mitochondria appear as smaller green circles with a red rim. There is matrix and membrane apparent in this image.
The detail available through our process will create new opportunities for molecular diagnosis of mitochondrial disease with only a traditional light microscope.
Phenomenal!
